bioprinting engineers

Abstract: Bioprinting is emerging as a tissue engineering technique to replace common treatment methods for large scale injuries. While thickness of the BioPrinted Constructs (BPCs) have shown to be of importance in the cell maturation and integration, the literature lacks a robust, automated, and quantitative method for measuring these metrics. In this paper, we propose a fully automated vision-based method for measuring the thickness of the BPCs with complex geometries. Leveraging the point cloud and RGB images of a structured light 3D camera, our proposed method performs an image-based segmentation for delineating the BPCs from the RGB images, accompanied by novel geometry-based thickness measurement algorithms performed on the point cloud scans. These algorithms combine the segmentation mask with the robot's forward kinematics data and a 3D point cloud scan to precisely measure the aforementioned metrics for complex-shaped BPCs. The proposed method was evaluated in simulation and experimental studies. In simulation studies, the algorithms were used to measure the thickness of some virtually created BPCs with known thickness. The comparison between the measured and true thicknesses demonstrates the high accuracy of the proposed method, achieving mean absolute errors between 0.025 mm and 0.057 mm in simulation at a spatial resolution of 0.1 mm x 0.1 mm per pixel. Furthermore, we successfully deployed the algorithms on our robotic bioprinting setup utilizing a structure light 3D camera, where complex patterns were printed and the developed methods utilized to accurately measure the thickness of printed BPCs.